What are the precautions for "12345 Citizen Hotline"?

Things to note

Things to note when calling the citizen service hotline:

1. The caller’s complaint must have clear facts and leave your real name and valid contact information;

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2. If the matter of appeal involves the scope of the Party Committee, People’s Congress, CPPCC, military, or judicial organs, citizens should directly report it to the petition and complaint departments of these units;

3. The matter of appeal has entered the judicial system For procedures (two trials or judgments by the court), citizens should report directly to the Standing Committee of the Municipal People's Congress, the Political and Legal Affairs Committee of the Municipal Party Committee, or the higher-level judicial authority; for emergency help involving the safety of life and property, citizens should directly dial 110, 119 and other emergency hotlines. ;

4. Parents should strengthen education and management of minors to prevent accidental calls; for invalid calls, agents will patiently persuade and persuade;

5. For those who make malicious harassing calls repeatedly despite being persuaded, the public security department will be held accountable in accordance with the relevant provisions of the "Public Security Management Punishment Law" upon verification.

Mainly accepted

1. Consultation on *** departments and staff responsibilities, service procedures, administrative approval, administrative acceptance, investment promotion and other policy regulations;

2. Opinions and suggestions on the city’s reform and opening up, economic construction, urban construction and management;

3. Needs for labor security, medical services, education, safety and other aspects of social life* ** demands for resolution;

4. Demands for *** coordination and resolution in terms of enterprise production and operation, development environment, etc.;

5. Requirements for *** departments and their work Criticisms, complaints, etc. from personnel. What are the precautions for hot air ballooning?

Hot air ballooning has a history of more than 200 years abroad, but it started late in China. Currently, there are only more than 120 hot air balloons in the country. Hot air balloons are expensive to build, each one costs about 80,000 to 100,000 yuan, and to fly a hot air balloon you need to obtain a "Hot Air Balloon Pilot's License" issued by the Civil Aviation Administration of China. Each flight not only requires the cooperation of multiple ground staff, but also requires filing in advance with the civil aviation management department and the air force command department. Even if you just go to the club to "experience", each 30-minute "free flight" costs 600 yuan, which is more expensive than playing golf.

Currently, in order to obtain the qualification to fly a hot air balloon in China, the tuition fee is about 16,000-18,000 yuan. After completing the course, if you are still flying in a professional club, you still need to pay a certain flight cost for each flight; the cost of a flight experience is about 400-500 yuan per hour; if you want to buy a hot air balloon, you can engage in flying in your own area For activities, you also need to apply for flight airspace approval and declaration at the air traffic control department. It costs about 76,000 yuan to buy a standard hot air balloon. In some tourist attractions in my country, there are also "tethered flights", that is, hot air balloons that are fixed and will not fly away. Although they can only rise to a height of 30 to 50 meters, you can still initially experience the feeling of "dominating the world" when flying hot air balloons.

Notes:

1. To prevent fire, pilots must wear cotton clothing. They cannot wear skirts, high heels, sandals, etc. They must wear sports shoes. And to prevent burns, be sure to wear long clothes, long pants, and a pure cotton baseball cap.

2. Patients with high blood pressure and heart disease are not allowed to perform hot air balloon exercises.

3. The best time to fly a hot air balloon is within two hours after sunrise or two hours before sunset, because the airflow is the most stable during this period. It is not suitable to fly a hot air balloon in windy seasons.

4. Each flight has a certain flight cost, which basically costs 400 to 500 yuan per hour.

5. The service life of a hot air balloon is basically 9 years, and annual inspections and sequential inspections are required.

6. Pay attention to cold protection when flying at high altitudes. For every 1,000 meters of altitude, the temperature drops by 6.5 degrees.

7. Professional flying requires a driving license issued by the Civil Aviation Administration of China.

8. In addition to the necessary equipment, you also need to wear a GPS global positioning system, an electronic compass, a walkie-talkie, a utility knife, a ZIPPO lighter, etc. What are the precautions for RT-PCR?

Some good habits in experiments

Before adding the reagent, mix it well to avoid uneven concentration after leaving it for a long time

Use up the pipette After that, it must be returned to the maximum measurement position to prevent the spring from losing its elasticity over time

Be sure to turn off the water bath and remember to remember

Discuss more with everyone, and pay more attention to the experiences discussed by others. , this is almost the shortcut to the fastest improvement

Prepare all the reagents yourself, so you can easily find the cause if there is a problem

Measures to prevent RNase contamination

1. All glassware should be dry baked at a high temperature of 180℃ for 6hr or more before use.

2. Plastic utensils can be soaked in 0.1% DEPC water or rinsed with chloroform (note: organic glass utensils cannot be used because they can be corroded by chloroform).

3. Plexiglas electrophoresis tanks, etc., can be washed with detergent first, rinsed with double distilled water, dried with ethanol, then soaked in 3% H2O2 at room temperature for 10 minutes, then rinsed with 0.1% DEPC water and allowed to dry. Dry.

4. The prepared solution should use 0.1% DEPC as much as possible and be treated at 37°C for more than 12 hours. Residual DEPC is then removed by autoclaving. Reagents that cannot be autoclaved should be prepared with DEPC-treated sterile double-distilled water, and then filtered and sterilized through a 0.22 μm filter membrane.

5. Operators wear disposable masks, hats, and gloves, and gloves must be changed frequently during the experiment.

6. Set up a dedicated laboratory for RNA operations, and all instruments should be dedicated.

2. Commonly used RNase inhibitors

1. Diethyl pyrophosphate (DEPC): It is a strong but incomplete RNase inhibitor. It denatures proteins by binding to the imidazole ring of histidine, the active group of RNase, thereby inhibiting enzyme activity.

2. Guanidine isothiocyanate: Currently considered the most effective RNase inhibitor, it lyses tissue while also inactivating RNase. It can not only destroy cell structures and dissociate nucleic acids from nuclear proteins, but also has a strong denaturing effect on RNase.

3. Vanadyl ribonucleoside complex: a complex formed by vanadium oxide ions and nucleosides. It combines with RNase to form a transition state substance, which can almost completely inhibit the activity of RNase.

4. RNase protein inhibitor (RNasin): an acidic glycoprotein extracted from rat liver or human placenta. RNasin is a non-competitive inhibitor of RNase and can bind to a variety of RNases to inactivate them.

5. Others: SDS, urea, diatomaceous earth, etc. also have a certain inhibitory effect on RNase.

Because DEPc indiscriminately modifies proteins and RNA, it cannot be used in the isolation and purification of RNA, and it is incompatible with some buffers (such as Tri)

The most critical factor in all RNA experiments is the isolation of full-length RNA. The main reason for the failure of the experiment was contamination by ribonuclease (RNase).

RNase can withstand a variety of treatments without being inactivated, such as boiling, autoclaving, etc.

Contamination by researchers The most significant potential source of contamination of RNase is the hands of researchers. Therefore, gloves should be changed frequently when conducting RNA experiments. What are the precautions for wide-mouth bottles, precautions for narrow-mouth bottles, and what are the uses and precautions for funnels?

Narrow-necked flask

A glass container used to store liquid reagents and therefore has a relatively small mouth. There are two types: transparent and brown. The brown bottle is used to hold reagents that need to be protected from light.

Precautions for use: cannot be used for heating. When taking reagents, put the bottle stopper upside down on the table, tighten it after use, and seal it if necessary. Since the inside of the bottle mouth is frosted and matched with a frosted glass stopper, it cannot hold strong alkaline reagents. If alkaline reagents are contained, a rubber stopper should be used instead.

Jars

Jars are glass containers used to hold solid reagents. There are two types: transparent and brown. Brown bottles are used to hold reagents that need to be protected from light. .

Precautions for use: cannot be used for heating. When taking reagents, place the bottle stopper upside down on the table, tighten it after use, and seal it if necessary. Since the inside of the bottle mouth is frosted and matched with a frosted glass stopper, jars with glass stoppers cannot hold strong alkaline reagents. If alkaline reagents are contained, what are the precautions for using rubber stoppers for loans

Loans through China Merchants Bank, the loan amount is small (less than 50,000), if you are already a China Merchants Bank user and have downloaded the Merchants Bank For the bank's mobile banking APP, you can try to log in to the mobile banking, click "My" - "All" - "Loan" - "I want to borrow money", and try to apply through this interface. If you meet the conditions for a CMB credit card, you can try to apply for a credit card.

If the loan amount is more than 50,000, if there is a China Merchants Bank in your city, you can try to apply for a loan through the China Merchants Bank branch. Since the conditions and application materials required for each loan project are different, the current loan uses are as follows: Legal personal or family consumption such as car purchase, parking space purchase, decoration, education and scholarships, bulk shopping, travel, etc.; as well as business turnover purposes. You can call 95555 from 8:30-18:00 to enter manual service and provide the loan purpose and city to learn more about the required information. Whether the loan application is approved shall be subject to the comprehensive review results of the personal loan department of the handling bank. What are the precautions for soldering?

1. The set temperature of the tin furnace should not be too high to avoid accelerated oxidation of the tin surface. It is generally set at around 300 degrees or according to actual use needs;

< p> 2. First put an appropriate amount of tin strip in the tin pot, connect the power, turn on the power switch, adjust the temperature to about 250 degrees, use a solder strip to apply tin on the red heating tube until the tin surface is covered and heated. Tube. When the tin bar begins to melt, the tin bar should be added in time until the molten tin surface reaches the appropriate height. Do not turn on electricity for heating when there is no solder in the tin furnace.

3. The temperature range of general tin furnace: 0-500 degrees. But the actual maximum temperature is: around 300-400 degrees;

Precautions when using the attached tin furnace

A. The tin furnace is connected to a ground wire, so the user must connect it and Ensure good grounding for safety;

B. Before using the tin furnace, check whether the power supply voltage is consistent;

C. The tin furnace should be kept dry and not exposed to moisture or rain. Work under conditions;

D. The tin furnace should be placed stably, and flammable items and other items should not be placed within 0.5m of the surrounding area;

E. Operators should use goggles and heat protection Gloves, be careful to avoid foreign objects falling into the melting tin pot during use to prevent accidents;

F. It is strictly forbidden to move after power is turned on, and do not knock, disassemble or install the electric heating parts at will;

< p> G. The shell has a temperature of 50℃-80℃ during use. This is normal. Pay attention to the high temperature and do not touch the shell;

H. Turn off the power after use and leave it unattended. Do not turn on the power to heat;

I. If a malfunction occurs, please ask personnel with professional maintenance skills to check and eliminate it. What are the precautions for blood transfusion?

(1) Before blood transfusion, the appearance of the whole blood must be strictly checked, whether the blood bag is damaged or leaking, and whether the blood color is qualified; the patient, cross-cooperation report form, and the blood to be transfused must also be carefully checked Whether it is correct, including the name, gender, ABO and Rho (D) blood type of the patient and blood donor, the results of the cross-match test and antiglobulin test, blood bag number, blood type and blood volume, etc., and it should be checked by two people, Blood transfusion can only be done accurately. (2) During blood transfusion, you should go to the patient's bedside to check the medical record number, patient name, blood type, etc. After confirming the recipient, use a standard blood transfusion set equipped with a filter (the filter pore size is approximately 170 microns, and the total effective filtration area is 24 -34 cm2, which can filter out aggregated platelets, white blood cells and fibrin that may be present in blood and blood component products for blood transfusion. (3) Blood should be taken out of the refrigerator before infusion and should not stay at room temperature for more than 30 minutes. Gently mix the blood in the blood bag before infusion to avoid violent shaking. No other drugs should be added to the blood. If dilution is needed, only intravenous saline can be used. (4) Flush the blood transfusion channels with normal saline before and after blood transfusion.

When continuously transfusing blood from different donors, after the previous bag of blood is exhausted, use intravenous injection to flush the blood transfusion device with normal saline, and then receive the next bag of blood to continue the transfusion. (5) The blood transfusion process should be slow at first and then fast, and then the transfusion speed should be adjusted according to the condition and age. The recipient should be closely observed for any adverse reactions of the transfusion. If any abnormality occurs, it should be dealt with in time. During the initial 10-15 minutes of blood transfusion or when the first 30-50 ml of blood is transfused, medical staff must closely monitor whether there are any adverse reactions. If an adverse reaction occurs, the blood transfusion must be stopped immediately and reported to the responsible physician for timely diagnosis and treatment. At the same time, the blood transfusion department or blood bank must be notified to conduct necessary cause investigation. Usually, blood does not need to be warmed for a transfusion. (6) After blood transfusion, keep the blood bag in a refrigerator at 2-8°C for 24 hours in case of accidents. (7) After the blood transfusion is completed, the medical staff will fill in the blood transfusion reaction investigation receipt item by item and return it to the blood transfusion department for storage the next day after the blood transfusion is completed. The blood transfusion department reports monthly statistics to the medical office (department), and the responsible physician records the blood transfusion situation in the medical record. (8) After the blood transfusion is completed, the medical staff will paste the second copy of the blood transfusion note in the medical record. What are the precautions for reversing?

When reversing, try to point the front of the car toward a place with a wide view and room to maneuver so that you can observe moving people or cars. Do not reverse in places where reversing is not allowed by traffic regulations to prevent accidents.

Expansion:

1. Introduction:

The behavior of a motor vehicle moving backward is reversing. It also refers to people "reversing" when traveling, that is, changing from one car to another.

2. Basic essentials

When entering a parking lot to park, first of all, visually check the width of the parking space, whether there are any obstacles behind it, whether there are cars parked on both sides, and whether the length matches your car type. . When you can't see clearly at night, it's best to come down and observe the exact position before pouring.

When reversing, try to reverse from the left (if conditions permit). The left side is convenient for the person reversing to observe. When reversing, you should first look behind you, and then pay attention to the rearview mirrors on both sides. The lateral distance on the right side is larger than on the left side. Because the turning radius of the front wheels is larger than the turning radius of the rear wheels, the front of the car will swing outward when reversing. If you don't pay attention to this, the right side will be easily scratched. The main speed during reversing is idling, and the vehicle body should be corrected in time. Each vehicle has different reference points and different timings for turning, so I won’t generalize here. [1]

Sometimes you park in a straight line along the shoulder of the road, often parking between two parked cars. This kind of reversing is difficult, so it is best to have someone directing you. When pouring, pay attention to the lateral distance from adjacent cars. When you see the rear of the car approaching the road shoulder in the rearview mirror, quickly turn your direction. Once the car is aligned, quickly turn back. When reversing, don't forget to keep the distance between you and the car behind you. If you don’t know the distance between cars well, just come down and take a look before falling back. During the reversing process, the steering speed should be fast so that the vehicle body can be straightened in a short time. Sometimes the vehicle body may not be properly positioned when moving forward or backward at the same time, and it may take several repetitions to park the car properly. What are the precautions for shooting?

The more classic shooting positions are lying, standing and kneeling. In special circumstances, squatting can be used. Here is a brief introduction to an exercise, the essentials of shooting from a lying position at a target that is not moving at 100 meters.

1. Posture.

After the shooter lies prone on the shooting mat and takes the ready position, the angle between his torso and the shooting direction projection is generally 10 to 20 degrees. The feet should be in an "outer shape" and close to the ground, and the left leg should be straight and approximately in a straight line with the left side of the body. Hold the gun with your left hand, the angle between the left forearm and the ground should be no less than 30 degrees, and the angle between the upper arm and the ground should be kept at about 45 degrees. The bottom of the butt of the gun should be against the right shoulder socket, the shoulder should be firmly pressed against the collarbone, and the right hand should hold the handle (gun neck). Press the right cheek tightly. When pressing the cheek, the gravity of the head is straight downward and the neck is relaxed. The force exerted by the entire body on the gun can only be felt forward and downward, but does not produce lateral push or pull force.

The height of the support depends on the individual's height and the length of the right arm. Generally, it is about 20-26 centimeters.

2. According to the gun.

When holding the gun, the right side of the body is slightly in line with the gun body. The right hand is holding the handle of the gun forward, the first section of the index finger is leaning on the trigger, and the right elbow is as close as possible to the ground. Hold the magazine in your left hand, place your left elbow on the ground, and keep both elbows stable. Keep your chest straight, your body slightly forward (right elbow does not leave the ground), your upper body naturally collapses, and the force of both hands remains unchanged, so that the butt of the gun is indeed pressed against the shoulder socket, and your face is slightly tilted forward, naturally touching your cheek.

The essentials of holding a gun can be summarized into ten words, namely: upright, holding, resisting, settling, collapsing, not pushing up and not pulling.

Upright: The right side of the body is basically aligned with the gun body, and the gun face should be straight.

Grip: Hold the right hand tightly with the tiger's mouth aligned, keep the grip unchanged when firing, and the right wrist should be closed, depressed, and held firm.

Resistance: The shoulder position is appropriate, tight and precise.

Fixed: Both elbows should be fixed.

Collapse: The upper body naturally collapses, keeping the posture of holding the gun stable.

Don’t push and don’t pull: means that after completing the action of holding the gun, keep the force unchanged and do not pull the gun backwards or push the shoulder forward.

3. Aim.

After locking the gun, arrange the upper edge of the notch, the upper edge of the front sight and the lower edge of the target in the same straight line. If you are not pointing at the target, cannot adapt and twist your body or change the action of holding the gun, you should adjust your posture or correct the supporting object. When correcting, you can move your body or elbows left and right. When correcting the height, you can move your body forward and backward or bring your elbows together. For external expansion, the support can also be adjusted appropriately. When aiming, your eyes should be looking at the target. If you focus on the crosshair, it may make the target blurry. The crosshair should be a black shadow with clear and sharp edges in your sight.

4. Firing.

The first joint of the right index finger should press the trigger evenly and straightly backwards (there should be a small gap between the inside of the index finger and the gun), and the strength of the remaining fingers should remain unchanged. As the sight line approaches the aiming point, begin to pre-trigger and breathe slowly. When the sight line points to the aiming point, you should stop breathing and continue to increase the pressure on the trigger until the trigger is fired. Correct and consistent aim should be maintained at the moment of firing. If the aiming line deviates from the aiming point or you cannot continue to stop breathing, you should neither increase nor relax the pressure on the trigger. After correction or ventilation, continue to press the trigger. Until the gun goes off, the shooting is completed. This is what is commonly said: "Consciously fire, unconsciously fire the gun."

5. Common problems.

1. Squeeze the trigger hard. It is easy to cause the gun body to swing, and most projectiles will miss the target or miss the target. The reason for slamming the trigger is due to the shooter's nervousness to capture the aiming point. This is the resulting weakness of a bad shooter.

2. Poor timing of firing. The performance is either too early or too late, often missing the opportunity, and there are many people who hesitate to fire.

In addition, when shooting, the main focus should be on the square relationship between the front sight and the gap. Always keep the front sight and the gap square. In other words, when shooting, you should look at the front sight and the gap is clear. And looking at the target is blurry.

The above are just some basic essentials of shooting. There are many reasons that affect shooting accuracy, including wind, sunlight, etc. However, as long as you master the correct shooting essentials, you can still overcome it. What are the precautions for notification?

A notification is an official document in which a superior informs a subordinate of relevant people and events. The notification has a wide scope of application and can be used by party and government agencies and units at all levels. Its function is to praise good people and good deeds, criticize mistakes and unhealthy tendencies, report serious incidents that should be taken as a warning, convey important situations and matters that need to be known to each unit. Its purpose is to exchange experiences, learn lessons, educate cadres and workers, and promote further work. The structure of the commendation (criticism) report has three parts: the first part explains the reasons for the commendation or criticism, that is, clearly describes the advanced deeds or wrong facts, and requires the use of narrative techniques to truly and objectively reflect the facts; the second part explains the narrative. Conduct accurate analysis and pertinent evaluation of the facts, without exaggerating or minimizing, so that people can be inspired by good people and things and learn from mistakes; the third part is generally a commendation or criticism Reward or punish for mistakes. Finally, based on the reported situation and the actual needs, a call or request must be made. The structure of the text of a situation report generally has two parts: one is the situation being reported, and the other is hopes and requirements.