2. When washing the sample tube and loading the solution, the glass slice and rubber washer should be kept well to prevent them from breaking or being lost;

3. When preparing sucrose solution, care should be taken to make the sucrose solids all dissolved and the solution fully mixed;

4. When determining α∞, care should be taken that the sample to be tested is not dissolved after 50 min of constant temperature at 50~60℃. When determining α∞, it should be noted that the sample to be measured in 50~60℃ condition after 50min constant temperature (but not more than 60℃, otherwise there is a side reaction occurs), move to the super thermostat and then constant temperature for 20min;

5. It is necessary to adjust the zero calibration of the rotameter, and if the adjustment can not be zero, it is necessary to carry out the data calibration.

Sucrose is a pure organic compound extracted from within the sugarcane and is one of the most closely related natural carbohydrates to life, which is produced by the combination of D-(-)-fructose and D-(+)-glucose through the combination of the hydroxyl groups of the hemiketone and hemiacetal. Acid hydrolysis of sucrose produces one molecule of D-glucose and one molecule of D-fructose

This reaction is a tertiary reaction, and in pure water the rate of this reaction is extremely slow, usually requiring the catalytic action of H+ ions. Since water is present in large quantities during the reaction, it can be approximated that the concentration of water is constant throughout the reaction, even though some of it is involved; and the concentration of H+, which is the catalyst, also remains constant. Thus the sucrose reaction can be viewed as a pseudo-primary reaction . Because sucrose and its transformation products are all cyclic, and they have different spinning ability, so you can use the system in the reaction process of the change in spin to measure the reaction process.